Zeiss LSM 900 With Airyscan-2 for Cryo-CLEM
Zeiss LSM900 at CMM is an upright confocal light microscope with Airyscan-2 for super-resolution imaging.
In combination with the Linkam CM196 Cryo-microscopy stage the system allows users to acquire high resolution fluorescent images of frozen hydrated cells that have been cultivated and cryo-fixed on EM sample carrier.
Fluorescent imaging under cryogenic conditions not only reduces fluorescence fading by a great amount, but also allows to directly transfer these cells into one of CMM’s cryo-transmission electron microscopes for cryoEM imaging.
Fluorescent labelled structures identified on the light microscopy level thus can be directly spatially correlated with high resolution cryo-EM images.
Available lenses:
| C Epiplan-Apochromat | 5x/0.2 DIC (FWD=21mm) |
| EC Epiplan-Neofluar | 10x/0.25 DIC (FWD=9.3mm) |
| Plan-Apochromat | 20x/0.8C (FWD=0.55mm) |
| C-Apochromat | 40x/1.2 W Corr (FWD=0.28mm at CG=0.17mm), UV-VIS-IR |
| C Plan-Apochromat | 63x/1.4 Oil DIC (FWD=0.14mm) |
| LD EC Epiplan-Neofluar | 100x/0.75 DIC (FWD=4.0mm) |
| EC Epiplan-Neofluar | 100x/0.9 DIC (FWD=1.0mm) |
Location: QBP Building 80
Contact: Matthias Floetenmeyer m.floetenmeyer@uq.edu.au