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CMM Image of the month winner!

May 2019

Image taken by Dr Sarah Piper from the Institute for Molecular Bioscience.

Title: Cryo-EM structure of the pore-forming toxin YenTcA

‘We have determined the cryo-EM structure of the bacterial pore-forming toxin YenTcA, a virulence factor produced by the insect pathogen Yersinia entomophaga, with data collected on the Tecnai F30 TEM. The image shows the single-particle cryo-EM map of the YenTcA protein complex in its pore conformation at 11 Å, as a side view (left) and top view (right), with the map coloured by different domains. In the background, a representative micrograph is shown, containing single particles of the YenTcA pore form in different orientations embedded in liposomes, on a holey-carbon grid. This data was recently published in Nature Communication .

The data was collected on the cryo-TEM Tecnai F30 microscope, equipped with a K2 direct electron detector, in the QBP CMM. The cryo-EM map was processed using the single-particle reconstruction software Relion and visualised using ChimeraX’.

CMM collects images to share online with the broader community. 

Please email if you have an image (along with a short description of the image and/or project) that you would be willing to donate to CMM.  We are looking for ‘high-res’ Scanning Electron Microscopy or Transmission Electron Microscopy images, Diffraction pattern, Maps,  raw or modified, any processed analytical data as well as 3D models.

We look forward to receiving your image (you are welcome to submit up to 3 images).  As a prize, each monthly winner will receive a Café Nano prepaid coffee card.


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