CMM Image of the month winner!
May 2019
Image taken by Dr Sarah Piper from the Institute for Molecular Bioscience.
Title: Cryo-EM structure of the pore-forming toxin YenTcA
‘We have determined the cryo-EM structure of the bacterial pore-forming toxin YenTcA, a virulence factor produced by the insect pathogen Yersinia entomophaga, with data collected on the Tecnai F30 TEM. The image shows the single-particle cryo-EM map of the YenTcA protein complex in its pore conformation at 11 Å, as a side view (left) and top view (right), with the map coloured by different domains. In the background, a representative micrograph is shown, containing single particles of the YenTcA pore form in different orientations embedded in liposomes, on a holey-carbon grid. This data was recently published in Nature Communication https://doi.org/10.1038/s41467-019-09890-8 .
The data was collected on the cryo-TEM Tecnai F30 microscope, equipped with a K2 direct electron detector, in the QBP CMM. The cryo-EM map was processed using the single-particle reconstruction software Relion and visualised using ChimeraX’.
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Please email cmm@uq.edu.au if you have an image (along with a short description of the image and/or project) that you would be willing to donate to CMM. We are looking for ‘high-res’ Scanning Electron Microscopy or Transmission Electron Microscopy images, Diffraction pattern, Maps, raw or modified, any processed analytical data as well as 3D models.
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