Olympus BX61 Upright Widefield Microscope
Principles:
This upright widefield microscope is fitted with brightfield, dark field, DIC, polarised light and fluorescence optics. The Olympus DP70 CCD camera is very sensitive to low fluorescence levels.
Suitable for:
Imaging fixed or risk group 1 biological material and soft matter
Imaging capabilities: DIC, Brightfield, Fluorescence (4 filters: UV, blue, red, yellow) and polarised light.
Benefits:
Manual stage
Slide stage holder
Olympus DP70 CCD camera that is very sensitive to low fluorescence levels.
Software: DP controller
Objectives:
| Position | Model | Magnification | NA | Immersion | Imaging Mode |
| 1 | Plan | 4x 10x | 0.1 0.3 | Dry Dry | BF, Polarised DIC, BF, DF, FL Polarised |
| 2 | UPlan Fl UPlan Fl | 20x 40x | 0.5 0.75 | Dry Dry | BF, DF, FL, Polarised DIC, BF, DF, FL, Polarised |
| 3 | UPlan Fl N | 60x | 0.9 | Dry | DIX, BF, DF, FL, Polarised |
| 4 | UPlan Fl C1 | 100x | 1.3 | Oil | BF, DF, FL, Polarised |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroicmatic mirror | Emission Filter | Suitable Dyes |
| 1 | U-MWU2 (MU) | 330-385 | 400 | 420 | DAPI, Hoechst |
| 2 | U-MWB2 (WB) | 460-490 | 500 | 520IF | GFP, Arcridine Orange, Nile Red |
| 3 | U-MWG2 (WG) | 510-550 | 570 | 590 | Cy3, AF568 |
| 4 | U-MWIY2 | 545-580 | 600 | 610IF | TxRed, mCherry, RFP, AF594, Cy5 |
| 5 | DIC |
Location: AIBN Building 75, Room 117
Contact: Nicole Schieber n.schieber@uq.edu.au