Nikon Eclipse Ti Inverted Fluorescence Microscope
Principles:
This is an inverted fluorescence microscope with a fully motorised stage and software integration.
Suitable for:
- Imaging fixed or risk group 1 biological material and soft matter
- Imaging capabilities: DIC, Brightfield, Fluorescence (5 filters: DAPI, CFP, GFP, YFP and TxRed)
Benefits:
- Motorised X-Y-Z stage
- Universal stage holder
- Epifluorescence illumination: Nikon intensilight C-HGFIE
- Nikon DS-Qi1Mc monochrome camera with Digital Sight DS-U3 controller
- Multichannel, mulit-point, z-stack and custom acquisitions
Objectives:
| Model | Magnification | NA | WD (mm) | Immersion | Imaging mode |
| Plan Fluor | 10x | 0.3 | 16 | Dry | DIC, BF, FL |
| Plan Fluor | 20x | 0.5 | 2.1 | Dry | DIC, BF, FL |
| Plan Fluor | 40x | 0.75 | 0.66 | Dry | DIC, BF, FL |
| Plan Apo | 40x | 1 | 0.12 | Oil | BF |
| Plan Apo VC | 60x | 1.4 | 0.13 | Oil | DIC, BF, FL |
| Plan Apo VC | 100x | 1.4 | 0.13 | Oil | DIC, BF, FL |
Fluorescent Filter Sets:
| Position | Name | Excitation | Emission | Suitable Dyes |
| 1 | DM400 | 340-380 | 435-485 | DAPI, Hoechst |
| 2 | CFP | 438/24 | 483/32 | CFP, Cerulean |
| 3 | DM505 | 450-490 | 520 | FITC, eGFP, AF488 |
| 4 | YFP | 500/24 | 542/27 | eYFP, mVenus |
| 5 | DM595 | 540-580 | 600-660 | TxRed |
| 6 | Empty |
Location: AIBN Building 75, Room 117
Contact: Nicole Schieber n.schieber@uq.edu.au