Nikon C2si Upright Confocal Microscope
Principles:
This microscope is an upright confocal with powerful and robust imaging capabilities. Images are high-quality with low-noise, and high-contrast thanks to high-efficiency scan heads and detectors, coupled with Nikon’s high-performance objectives. Two options of spectral imaging: fast and accurate 32-channel spectral detector or high-sensitivity GaAsP detector for tuneable, user defined emission bandwidth ranges.
Suitable for:
- Imaging fixed or risk group 1 biological material and soft matter
- Imaging capabilities: DIC, Brightfield, Fluorescence (3 filters: DAPI, FITC and TxRed) and Confocal spectral imaging within 400-720nm range
- No camera for widefield and fluorescent images but detectors provide high-quality confocal images
Benefits:
- Fully motorised X-Y-Z stage; z-step 0.025µm
- Tiled imaging
- Multi-position imaging
- Spectral imaging - fast and accurate 32-channel spectral detector or high-sensitivity GaAsP detector
- Intelligent, user-friendly software that is capable of programming complex imaging regimes - NIS Elements AR (advanced research) imaging and analysis software
Objectives:
| Position | Model | Magnification | NA | WD (mm) | Immersion | Imaging mode |
| 1 | Plan Apo λ | 10x | 0.45 | 4.0 | Dry | BF, FL |
| 2 | Plan Apo λ | 20x | 0.75 | 1.0 | Dry | BF, DIC, FL |
| 3 | Plan Apo λ | 40x | 0.95 | 0.25-0.17 | Dry | BF, DIC, FL |
| 4 | TU Plan Apo | 50x | 0.80 | 2.0 | Dry | BF |
| 5 | Plan Apo λ | 60x | 1.40 | 0.13 | Oil | BF, DIC, FL |
| TU Plan ELWD | 100x | 0.80 | 4.5 | Dry | BF, long WD, limited FL* | |
| * transmission rate for 405nm-436nm is around 50%, 436-656nm is around 70% | ||||||
Lasers:
LU-N4/LU-N4S laser unit: a compact laser unit pre-installed with four lasers - 405 nm, 488 nm, 561nm, 640 nm.
2 options for spectral imaging:
C2-DUS Spectral Detection Unit
- Allows acquisition of 32 channels of fluorescence spectra over a 320nm wavelength range with a single scan
- Number of channels: 32,
- Wavelength detection range: 400 - 750 nm,
- Spectral image acquisition speed: 2 fps (256 x 256 pixels)
- Maximum pixel size: 1024 x 1024 (Spectral mode/Virtual filter mode), Wavelength resolution: 2.5/5.0/10.0 nm,
- Wavelength range variable in 0.25 nm steps
C2-DUVB GaAsP detector unit
- Fully tuneable emission detector unit capable of spectral imaging with user defined emission bandwidths in Galvano imaging modalities.
- Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (C2-DUVB-OP) with a user-defined dichroic mirror and barrier filter,
- Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest: 320 nm,
- Maximum pixel size: 2048 x 2048 (CB mode/VB mode),
- Wavelength resolution: 10 nm,
- Wavelength range variable in 1 nm steps
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic Emission | Suitable Dyes |
| 2 | DM415 | 362-396 | 432-8 | DAPI, Hoechst |
| 3 | DM505 | 465-495 | 512-558 | FITC, eGFP, AF488 |
| 4 | DM595 | 540-580 | 600-660 | TxRed, mCherry |
Location: AIBN Building 75, Room 114
Contact: Nicole Schieber n.schieber@uq.edu.au