An Automated Freeze Substitution (AFS) machine is used to dehydrate and embed samples in plastic that have previously been cryo-fixed in a high pressure freezer. During pre-programmed steps with increasing temperatures (starting at -90 deg) water is slowly replaced by acetone and later by a liquid resin. The resin is then at a final step cured either at 60 deg C or at -40deg under UV-light. Only this solidification allows to section the samples with an ultramicrotome down to a thicknesse of 30nm.

Location: QBP Building

Contact: Dr Matthias Floetenmeyer on m.floetenmeyer@uq.edu.au

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