Leica EM AFS2, Automatic Freeze-Substitution Unit
An Automated Freeze Substitution (AFS) machine is used to dehydrate and embed samples in plastic that have previously been cryo-fixed in a high pressure freezer. During pre-programmed steps with increasing temperatures (starting at -90 deg) water is slowly replaced by acetone and later by a liquid resin. The resin is then at a final step cured either at 60 deg C or at -40deg under UV-light. Only this solidification allows to section the samples with an ultramicrotome down to a thicknesse of 30nm.
Location: QBP Building
Contact: Dr Matthias Floetenmeyer on email@example.com