Introductory TEM Training Course

Learn the theory and application of a Transmission Electron Microscope (TEM) to collect images from your sample and assist in your research.

This course provides a comprehensive introduction to Transmission Electron Microscopy (TEM) across all fields of research at UQ, from Materials to Biological science. During this course you will be introduced to the fundamentals of TEM as well as its practical application. 

This TEM training is hands-on and designed to get you imaging your samples straight away.  To make the most of your efforts you need to have your samples ready and be confident in preparing them on TEM grids.  If you're still in the preparation stage, contact Nicole Schieber to plan the best timing for your training.

Learning Objectives

What will you learn (+)

• How to practically operate the Hitachi HT7700 A and B at a basic level.
• An understanding of the theoretical fundamentals of Transmission Electron Microscopy (TEM).

Course Format

TEM training courses are offered regularly and include approximately 12 hours of participation time.

Part 1: Sample preparation for TEM

TEM requires samples to be prepared on to TEM grids for imaging. Depending on the nature of your sample, for example, biological, soft matter, nanoparticles or materials samples, the method can be different. Within CMM, we have experience across many sample types and can guide researchers in the best approach. See CMM Techniques for more information. This needs to be completed prior to starting your TEM training. If you do not have your samples on a TEM grid and need help to prepare your sample, please reach out to Nicole Schieber for assistance in this process. Participation time varies according to method.  

For a small number of samples (5-10), it may be worth considering fee for service, where we do the work for you. This is a service that is offered at CMM and the work is charged per hour by calculating the instrument and staff time. Explained here. 

Part 2: TEM Introduction Lecture

AIBN (Bldg 75) Level 1 Seminar Room, 9am - 11am Friday. Offered every two months. 

This lecture will cover the theoretical background of TEM and provide an overview of the essential operation of a TEM. It will cover the following topics: an introduction to the electron and its properties, electron-matter interactions, the illumination system including, electron guns, electromagnetic lenses, apertures and lens aberrations, the imaging system, contrast mechanisms, and operator control of the instrument. 

The lecture will be followed by a discussion with new clients on their project, their requirements, and expectations. 

Part 3: Self-directed, at home learning

Learning material is supplied following attendance to the Theory Lecture, it can be completed in your own time but must be finalised before moving to practical training (part 4). Participation time is approximately four hours. Competency of this portion is assessed by quiz questions. 

Check out the MyScope TEM module to get a head start!

Part 4: TEM Practical Training

AIBN (Bldg 75) Level 1 Room 112 

2 Sessions daily: 10am - 12pm or 1pm – 3pm, over Monday - Wednesday. Offered monthly.  

This part of the course involves hands-on practical training at the TEM working 1:1 with you and your samples. Entry is dependent on availability and completion the part three self-directed learning material. You must have your own samples prepared on TEM grids in advance. 

During these sessions, your trainer will individually assess your progress, and when you have demonstrated competency in a test, you will be given the opportunity to book with CMM staff for an assisted session in the following two weeks. At this session, CMM staff will observe your capabilities at the TEM and offer advice where needed. It is at the discretion of CMM staff whether you will be given a 3-month license on the instrument or need further supervised sessions. Once you have your 3-month license, you are required to complete 10 hours of independent microscope operation for your license to be extended to 12 months. If you have not returned within the 3 months following your training or have less than 10 hours of independent microscope use, you must repeat the competency test in an assisted microscope session. Please note that the training credit will be applied (explanation below); this is our way to ensure you consolidate the training in the months following the initial training. 

Part 5: Independent Microscope Operation

You’ve done the hard work – now it’s time to apply it! The best way to build confidence and skill is to get hands-on. Training is just the beginning. Using the TEM on your own is where the real learning happens – and we’re here to help if you need guidance as you go. 

We recommend a minimum of 10 hours independent microscope operation following your training, which coincides with your training credit. 

Upgrade trainings can be arranged when necessary for diffraction, EDS or HF5000 once you have met the prerequisites (instrument time and advanced competency). 

Training Credit

If you are undertaking your first block training course in SEM and TEM then you must commit to a training and skills enhancement credit fee of $460. The credit will include 10 hours of instrument access time and can be used for your research project to enhance your knowledge.  The credit is available for 3 months from your initial training and is not transferable to other users or techniques. Any questions on the training course fee contact cmm@uq.edu.au.

Upgrade Training

Upgrade training can be arranged when necessary for diffraction, EDS or HF5000 once you have met the prerequisites.

Timetable 2025

Location and time: 

Lecture: AIBN (Bldg 75) Level 1 Seminar Room, 9am - 11am 

Practical training: AIBN (Bldg 75), Level 1, Room 112, CMM Life Science and Soft Matter Lab. Mornings 10 am – 12 pm / Afternoons 1 pm – 3 pm. 

The dates for 2025 TEM practical training and the lecture introducing the fundamentals of TEM are listed below.

Related courses

There are many other CMM Techniques available which may be useful in your research.  Here is a list of related courses: 

• Sample Preparation for Biological Science, Polymer Sciences and General Morphology 
• Sample Preparation for Materials and Physical Sciences 
• Grid Coating 
• Negative Staining 
• Ultramicrotomy 
• TEM – Upgrade Training 
• CryoTEM – Upgrade Training 

Banner Image by Dr. Erica Lovas, CMM Staff Scientist: Rock-boring Caribbeam Clam Gastrochaena ovata – sperm. 

Upgrade TEM training table image published in Jingqi Zhang et al. (2024). Ultrauniform, strong, and ductile 3D-printed titanium alloy through bifunctional alloy design. Science.