1: Negative Stain (group training 3 people maximum)
Training package $350 AUD. Includes training + 10 hours on the microscope post training. JEOL 1400 flash fees are $34.50 AUD, 4 hours on MP post training.
Condition of entry: need to use the TEM independently at least once every second week or have more than 5 samples to process.
6 Hours training on room temperature TEM (JEOL 1400 Flash)
- Independency test at the end of the 6 hours.
- Provisory license for 3 months: need to have spent at least 10 hours on the instrument by the 3 month limit and pass a second independency test to access full License.
- Full License: must be renewed every 6 months, receive after hours access on the JEOL 1400 Flash.
Optional - Mass-photometry 2 hours
Provisory License for 3 months. Extended to full 6 month license if you have spent 4 hours on the machine.
2. Cryo EM Training (group training 3 people maximum)
Training package $350 AUD. Includes training plus 2 x 8 hours assisted session on the microscope post training (8 hours during peak time $46 AUD/hour).
Condition of entry: need to have the full negative stain license, revision of results from negative staining and mass-photometry to make sure the sample is ready for cryoTEM.
Week 1:
Tuesday: 2.5 hours plunge freeze training on Leica EM GP2
Thursday: 2.0 hours plunge freeze training on Vitrobot Mark IV
Week 2:
Tuesday: 6 hours cryoTEM training on Cryo ARM 200 (focus on cryoEM green screening)
Thursday: 6 hours cryoTEM Training on Cryo ARM 200 (focus on cryoEM automatic data collection)
- Independency test at the end of the second day
- Provisory license for 3 months: need to have spent at least 2 sessions of 24 hours on the instrument by the 3 month limit and pass a second independency test to access full license.
- Full license: must be renewed every 6 months, receive after-hours access on the cryoARM 200 and cryoARM300 and access to ThermoFisher glacios training.
3. Cryo EM Thermofisher glacios 2 training (individual training only)
Condition of entry: need to have the full cryoEM license
Day 1: Wednesday afternoon - auto-grid clipping training.
Day 2: Thursday full day - 6 hours training on the Glacios 2 and EPU software.
- No independency test as it is an upgrade training
- Full license given after training as already an independent user on 1400 and cryoARMs. Must be renewed every 6 months, receive after-hours access on ThermoFisher glacios.
4. Pre-Training
Launch RIMS project
In-person meeting
- more than 5 samples
- ready to use the instrument in less than 1 month
Lecture:
- 2 Hours Basic TEM
- 1 Hour CryoTEM specific lecture
Home work:
- MyScope + assessment
- CryoTEM specific quiz
Enquiries contact Lou Brillault; l.brillault@uq.edu.au
Visual recap
